This platform offers the ability to generate sequences up to and including 150 Kb in length with one hundred percent accuracy, guaranteed to be identical to the sequence requested by the customer.Ī commonly used method of DNA fragment assembly is through the use of a yeast intermediate. This process has been perfected by Synbio Technologies through our three phase Syno Platform. This process is then repeated until the required sequence length is generated. These single stranded molecules are then enzymatically assembled into larger molecules. Today, all DNA synthesis methods begin with solid-phase phosphoramidite chemistry to construct single-stranded DNA molecules ranging in size between 10 and 100 base pairs in length. Although clonal plasmid-based intermediates might exist during the assembly of a target DNA, every base originates as a phosphoramidite molecule at the beginning of DNA synthesis. With gene synthesis we, at Synbio Technologies, are capable of generating de novo genetic sequences that may not currently be present anywhere within nature. This step deviates from the traditional method of studying genetics, by which a preexisting genetic sequence was extracted from an organism to be amplified and later studied. The essential feature of DNA synthesis is that no naturally isolated DNA is used. Applications and Research of Gene TherapyĭNA synthesis is defined as the process by which copies of nucleic acids are strung together to form a longer DNA sequence within a laboratory setting.ĭNA oligomers are the foundation of the DNA synthesis process.Target Screening for New Drug Development.
Ultra-long Genome Synthesis and Assembly.Codon Optimization for Peptide and Protein Expression.Applications of Gene Synthesis on the Syno ® Platform.Error-prone scFv Variant Antibody Library.Synthetic CDRs Variant Antibody Library.Livestock and Poultry Genome CRISPR Knockout Libraries.
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